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      <image:caption>Produced by the National Institute of Allergy and Infectious Diseases (NIAID), this photomicrographic montage was created by combining two slides, processed using the immunofluorescent antibody technique (IFA). One slide was used to identify spirochetes that express outer surface protein D, resulting in yellow, and red-colored organisms. Again, using IFA on the second slide, spirochetes were labeled with an anti-B. burgdorferi antibody, producing organisms that had stained a glowing green color. The Lyme disease spirochete, B. burgdorferi, is an obligate parasite that cycles between ticks and vertebrate hosts. B. burgdorferi alters the proteins expressed on its outer surface, depending on the state of each host. Therefore, by using two different specimens, and staining the specimens in accordance with these two protein coat configurations, both states are combined into a single image.</image:caption>
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      <image:caption>This 2007 photograph depicts the pathognomonic erythematous rash in the pattern of a bull’s-eye, referred to as erythema migrans. The rash manifested at the site of a tick bite, on this Maryland woman’s posterior upper arm, signifying a case of Lyme disease, caused by the bacterium Borrelia burgdorferi, and transmitted to humans, by the bite of infected blacklegged ticks.</image:caption>
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      <image:caption>This scanning electron microscopic (SEM) image depicts a grouping of numerous Gram-negative, anaerobic, Borrelia burgdorferi bacteria, which had been derived from a pure culture. See PHIL 13167 for a colorized version of this image. This pathogenic organism is responsible for causing the illness, Lyme disease, a zoonotic, vector-borne, ailment, transmitted to humans by way of a tick bite. For a closer view of these organisms, see PHIL 13166.</image:caption>
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      <image:caption>This image depicts the posterior knee, or popliteal region of a patient’s right leg who had presented with an erythema migrans (EM) rash, characteristic of what was diagnosed as Lyme disease, caused by the bacterium, Borrelia burgdorferi. B. burgdorferi bacteria are transmitted to humans through the bite of infected blacklegged ticks.</image:caption>
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      <image:caption>Under a magnification of 158X, this photomicrograph of a Warthin-Starry stained heart tissue specimen, revealed the presence of a number of Borrelia burgdorferi spirochetes, the cause of Lyme disease. At this stage, after having infected the heart muscle, the disease is more specifically referred to as Lyme carditis.</image:caption>
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